DNA Fingerprints of Two Tilapia Fish Species of Euphrates River at Governorate of Al-Muthanna Using RAPD Markers
AbstractDuring the last decade, tilapia species (Cichlidae: Teleostei) became members of Iraqi fish fauna. They characterized with morphological similarity among genera and species. That makes species differentiation not easily. Molecular methods followed to differentiate between redbelly tilapia Coptodon zillii (Gervais, 1848) and blue tilapia Oreochromis aureus (Steindachner, 1864) of the Euphrates River sector at Al-Samawah city. RAPD-PCR method used to create the genetic fingerprints of two tilapia fish species. Seven decamer primers (OPA08, OPA10, OPA13, OPA17, OPA19, OPB08 and OPC02) used to amplify DNA fragments using PCR-RAPD technique. Forty-four bands scored after electrophoresis on 2% agarose gel along with molecular marker fragmented to each 100 base pair. The molecular weight of bands was calculated using PhotoCapt-MW software. The volume of bands ranged from C. zillii 168 bp to 2227 bp while they ranged from 62 bp to 2154 bp in O. aureus. The results achieve the RAPD fingerprints of two tilapia species in Euphrates River at for genetic Al-Samawa city and draw the genetic tree with the same species from Shatt A-Arab River in Basrah city. The study concluded that there is the closest relatedness among tilapia populations from Euphrates and Shatt Al-Arab Rivers. The results proved that RAPD markers were efficient to generate DNA fingerprints of tilapia fish species. Furthermore, the utilizing of the RAPD markers can differentiate the two studied species. The present study may be the first genetic study on these tilapia fish species. Moreover, this would be the baseline studies in the future.
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